Cytokeratin 17 Polyklonaler Antikörper

Cytokeratin 17 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18502-1-AP

Synonyme

39.1, Cell and organelle markers, CK 17, Cytokeratin 17, Cytoskeleton Marker, K17, keratin 17, KRT17, PCHC1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 18502-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 18502-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18502-1-AP

  IP Result of anti-Cytokeratin 17 (IP:18502-1-AP, 4ug; Detection:18502-1-AP 1:600) with HeLa cells lysate 1600ug.

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  IHC staining of human cervical cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human cervical cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18502-1-AP (Cytokeratin 17 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18502-1-AP (Cytokeratin 17 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 18502-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 17 antibody (18502-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 18502-1-AP

  Immunofluorescent analysis of HeLa cells using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 18502-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Cytokeratin 17 antibody (18502-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Hautgewebe, humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18502-1-AP bindet in WB, IP, IHC, IF, FC, ELISA Cytokeratin 17 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 17 fusion protein Ag13351
• Vollständiger Name: keratin 17
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC011901
• Gene symbol: KRT17
• Gene ID (NCBI): 3872
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filaments that make up the cytoskeleton. Type I keratins are a group of acidic intermediate filament proteins and type II keratins are the basic or neutral counterparts. Keratin 17 is encoded by the KRT17 gene and is a type I cytokeratin found in nail beds, hair follicles, sebaceous glands, and epidermal appendages.

 What is the molecular weight of cytokeratin 17?

The molecular weight of cytokeratin 17 is approximately 49 kDa.

 What is the function of cytokeratin 17?

Cytokeratin 17 plays a role in the formation and maintenance of epidermal appendages, especially in determining the shape and orientation of hair. It is required for the maintenance of the anagen (growth) state of hair follicles by modulating the function of TNF-alpha for hair cycling and is also involved in tissue repair (PMID: 16702408).

 What is the cellular localization of cytokeratin 17?

Cytokeratin 17 expression is located in the cytoplasm.

 What is the tissue specificity of cytokeratin 17?

Cytokeratin 17 expression is found in the outer root sheath and medulla regions of the hair follicle, particularly in the eyebrow, beard, nail bed epithelium, basal cells of the oral epithelium, and sweat and mammary glands (PMID: 2451629). Expression can also be found in epithelial layers of the prostate and urinary bladder and cambial cells of the sebaceous gland at the protein level.

 What is the role of cytokeratin 17 in disease?

Mutations in the KRT17 gene are a cause of Jackson-Lawler type pachyonychia congenita type 2 (PC2); an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy (PMID: 7539673). Defects in KRT17 also lead to steatocystoma multiplex (SM) (PMID: 9767294). Cytokeratin 17 acts as an autoantigen in the immunopathogenesis of psoriasis (PMID: 23722817).