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Cytokeratin 18 Polyklonaler Antikörper
Cytokeratin 18 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (5)
Anwendung
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 10830-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | A431-Zellen, HeLa-Zellen, K-562-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen, Mauslebergewebe, Rattenlebergewebe |
Erfolgreiche IP | A431-Zellen |
Erfolgreiche Detektion in IHC | humanes Mammakarzinomgewebe, humanes Appendizitis-Gewebe, humanes Kolonkarzinomgewebe, humanes Endometriumkarzinomgewebe, humanes Nierengewebe, humanes Leberkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Ovarialkarzinomgewebe, humanes Pankreaskarzinomgewebe, humanes Prostatahyperplasie-Gewebe, humanes Nierenzellkarzinomgewebe, humanes Magenkrebsgewebe, humanes Tonsillitisgewebe, Mauslebergewebe, Rattenlebergewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | HepG2-Zellen, HeLa-Zellen |
Erfolgreiche Detektion in FC (Intra) | HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:20000-1:100000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:200-1:1000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 40 publications below |
IHC | See 27 publications below |
IF | See 62 publications below |
Produktinformation
10830-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA Cytokeratin 18 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Affe, Ente, Hausschwein, Maus, Ratte, Rind, Ziege |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Cytokeratin 18 fusion protein Ag1260 |
Vollständiger Name | keratin 18 |
Berechnetes Molekulargewicht | 48 kDa |
Beobachtetes Molekulargewicht | 48 kDa |
GenBank-Zugangsnummer | BC000180 |
Gene symbol | Cytokeratin 18 |
Gene ID (NCBI) | 3875 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for Cytokeratin 18 antibody 10830-1-AP | Protokoll herunterladen |
IHC protocol for Cytokeratin 18 antibody 10830-1-AP | Protokoll herunterladenl |
IF protocol for Cytokeratin 18 antibody 10830-1-AP | Protokoll herunterladen |
IP protocol for Cytokeratin 18 antibody 10830-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Cell Stem Cell Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency. | ||
Hepatology Fat-associated Lymphoid Clusters as Expandable Niches for Ectopic Liver Development. | ||
J Control Release Mesenchymal stem cell exosomes as nanotherapeutics for dry age-related macular degeneration | ||
Acta Biomater A KPV-binding double-network hydrogel restores gut mucosal barrier in an inflamed colon. | ||
Proc Natl Acad Sci U S A Role of estrogen receptor beta in uterine stroma and epithelium: Insights from estrogen receptor beta-/- mice. | ||
Proc Natl Acad Sci U S A Basal epithelial stem cells are efficient targets for prostate cancer initiation. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Jan (Verified Customer) (11-20-2024) | Photo attached represents IF analysis of fixed A549 cells. Cells were incubated with Cytokeratin 18 polyclonal antibody (10830-1-AP) and FITC-conjugated Goat Anti-Rabbit IgG (H+L) (SA00003-2).
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FH Parmveer (Verified Customer) (12-02-2019) | Works well for both IHC and IF, but does come with a bit of background
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