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Cytokeratin 18 Polyklonaler Antikörper

Cytokeratin 18 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (5)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(125)

Kat-Nr. : 10830-1-AP

Synonyme

KRT18, Cell proliferation-inducing gene 46 protein, CK 18, CK18, CK-18



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA431-Zellen, HeLa-Zellen, K-562-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen, Mauslebergewebe, Rattenlebergewebe
Erfolgreiche IPA431-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe, humanes Appendizitis-Gewebe, humanes Kolonkarzinomgewebe, humanes Endometriumkarzinomgewebe, humanes Nierengewebe, humanes Leberkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Ovarialkarzinomgewebe, humanes Pankreaskarzinomgewebe, humanes Prostatahyperplasie-Gewebe, humanes Nierenzellkarzinomgewebe, humanes Magenkrebsgewebe, humanes Tonsillitisgewebe, Mauslebergewebe, Rattenlebergewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen, HeLa-Zellen
Erfolgreiche Detektion in FC (Intra)HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:20000-1:100000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:200-1:1000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

10830-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA Cytokeratin 18 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Affe, Ente, Hausschwein, Maus, Ratte, Rind, Ziege
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Cytokeratin 18 fusion protein Ag1260
Vollständiger Name keratin 18
Berechnetes Molekulargewicht 48 kDa
Beobachtetes Molekulargewicht 48 kDa
GenBank-ZugangsnummerBC000180
Gene symbol Cytokeratin 18
Gene ID (NCBI) 3875
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Cytokeratin 18 antibody 10830-1-APProtokoll herunterladen
IHC protocol for Cytokeratin 18 antibody 10830-1-APProtokoll herunterladenl
IF protocol for Cytokeratin 18 antibody 10830-1-APProtokoll herunterladen
IP protocol for Cytokeratin 18 antibody 10830-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIHC

Cell Stem Cell

Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency.

Authors - Brian Lin
mouseIF

Hepatology

Fat-associated Lymphoid Clusters as Expandable Niches for Ectopic Liver Development.

Authors - Bing Han
ratIHC

J Control Release

Mesenchymal stem cell exosomes as nanotherapeutics for dry age-related macular degeneration

Authors - Yue Tang
ratIF

Acta Biomater

A KPV-binding double-network hydrogel restores gut mucosal barrier in an inflamed colon.

Authors - Yingzheng Zhao
mouseIHC,IF

Proc Natl Acad Sci U S A

Role of estrogen receptor beta in uterine stroma and epithelium: Insights from estrogen receptor beta-/- mice.

Authors - Wada-Hiraike Osamu O
mouseIF

Proc Natl Acad Sci U S A

Basal epithelial stem cells are efficient targets for prostate cancer initiation.

Authors - Lawson Devon A DA

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Jan (Verified Customer) (11-20-2024)

Photo attached represents IF analysis of fixed A549 cells. Cells were incubated with Cytokeratin 18 polyclonal antibody (10830-1-AP) and FITC-conjugated Goat Anti-Rabbit IgG (H+L) (SA00003-2).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: A549
Cytokeratin 18 Antibody Immunofluorescence validation (1:200 dilution) in A549 (Cat no:10830-1-AP)
FH

Parmveer (Verified Customer) (12-02-2019)

Works well for both IHC and IF, but does come with a bit of background

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Prostate
Cytokeratin 18 Antibody Immunohistochemistry,Immunofluorescence, validation (1:1000 dilution) in Prostate (Cat no:10830-1-AP)