Cytokeratin 19 Polyklonaler Antikörper

Cytokeratin 19 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (5)

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(162)

Rezensionen (2)

Kat-Nr. : 10712-1-AP

Synonyme

CK 19, CK19, KRT19, CK-19, Cytokeratin,Keratin

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  WB analysis using 10712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10712-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10712-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 10712-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse skin using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of Retinal organoids using 10712-1-AP

  Retinal organoids (day 60) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and Cytokeratin 19 using 10712-1-AP at 1:200 (red). Nuclear stain DAPI (blue). Scale bar = 100 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

• 

  IF Staining of mouse pancreas using 10712-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse pancreas using 10712-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HaCaT using 10712-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10712-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10712-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 10712-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 10712-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug Cytokeratin 19 Polyclonal antibody (10712-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, HepG2-Zellen, HT-29-Zellen, MCF-7-Zellen, Maushirngewebe, Maus-Plazenta-Gewebe, T-47D-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Mammakarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Ösophaguskarzinomgewebe, humanes Pankreasgewebe, humanes Hautgewebe, humanes Magenkrebsgewebe, humanes Schilddrüsenkarzinomgewebe, Maus-Kolongewebe, Maus-Pankreasgewebe, Maushautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Retinal organoids, Maus-Pankreasgewebe
• Erfolgreiche Detektion in IF/ICC: HaCaT-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunhistochemie (IHC): IHC : 1:10000-1:40000
• Immunfluoreszenz (IF)-P: IF-P : 1:10-1:100
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 29 publications below
• IHC: See 75 publications below
• IF: See 82 publications below

Produktinformation

10712-1-AP bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Cytokeratin 19 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, hund, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 19 fusion protein Ag1085
• Vollständiger Name: keratin 19
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 44-50 kDa
• GenBank-Zugangsnummer: BC007628
• Gene symbol: Cytokeratin 19
• Gene ID (NCBI): 3880
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytokeratin 19 (CK19 or KRT19) is a type I (acidic) cytokeratin. It is an intermediate filament protein providing structural rigidity and multipurpose scaffolds in epithelial cells. CK19 is often overexpressed in various cancers (e.g., hepatocellular carcinoma [HCC], pancreatic adenocarcinoma, lung cancer) and serves as a biomarker for hepatic progenitor cells (HPCs) associated with poor prognosis in HCC patients . Additionally, CK19 expression is common in pancreatic and gastrointestinal adenocarcinomasand has been studied as a potential diagnostic and prognostic marker for pancreatic neuroendocrine tumors (PNETs), where positive CK19 expression correlates with poor prognosis. Serum CK19 fragments (e.g., CYFRA 21-1, CK19-2G2) have been investigated as tumor markers for lung and breast cancer, with preoperative levels associated with metastasis and survival.

Publikationen

Species	Application	Title
mouse	IF	ACS Nano; Inflammation and Acinar Cell Dual-Targeting Nanomedicines for Synergistic Treatment of Acute Pancreatitis via Ca2+ Homeostasis Regulation and Pancreas Autodigestion Inhibition; Authors - Yanan Wang
mouse	IHC,IF	Nat Commun; Interventional hydrogel microsphere vaccine as an immune amplifier for activated antitumour immunity after ablation therapy; Authors - Xiaoyu Liu
human	IHC	Sci Transl Med; PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.; Authors - Tian-Yi Jiang
human		Adv Sci (Weinh); PMID: 31728273; Authors - Qingming Tang
human		Nat Commun; Reversal of pancreatic desmoplasia by re-educating stellate cells with a tumour microenvironment-activated nanosystem.; Authors - Xuexiang Han
human	IHC	J Clin Invest; CCN1 induces hepatic ductular reaction through integrin αvβ5-mediated activation of NF-κB.; Authors - Ki-Hyun Kim

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kis (Verified Customer) (02-21-2025)

got nice results in IF analysis in mouse live tissues sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300

FH

Alessandro (Verified Customer) (07-27-2022)

No aspecific staining, great outcome

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: ROs