Cytokeratin 19 Polyklonaler Antikörper

Cytokeratin 19 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (4)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

120

Rezensionen (1)

Kat-Nr. : 10712-1-AP

Synonyme

Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19

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Galerie der Validierungsdaten

• 

  WB analysis using 10712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10712-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 10712-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10712-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10712-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10712-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10712-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse skin using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse colon using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Retinal organoids using 10712-1-AP

  Retinal organoids (day 60) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and Cytokeratin 19 using 10712-1-AP at 1:200 (red). Nuclear stain DAPI (blue). Scale bar = 100 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

• 

  IF Staining of HaCaT using 10712-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10712-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 10712-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10712-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HT-29 using 10712-1-AP

  1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 19 (10712-1-AP) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, HepG2-Zellen, HT-29-Zellen, Maushirngewebe, Maus-Plazenta-Gewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Hautgewebe, humanes Lungenkarzinomgewebe, humanes Magenkrebsgewebe, humanes Mammakarzinomgewebe, humanes Ösophaguskarzinomgewebe, humanes Pankreasgewebe, humanes Schilddrüsenkarzinomgewebe, Maushautgewebe, Maus-Kolongewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HaCaT-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC: HT-29-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:3000-1:30000
• Immunhistochemie (IHC): IHC : 1:3000-1:12000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 23 publications below
• IHC: See 58 publications below
• IF: See 59 publications below
• FC: See 1 publications below

Produktinformation

10712-1-AP bindet in WB, IHC, IF, FC, ELISA Cytokeratin 19 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, hund, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 19 fusion protein Ag1085
• Vollständiger Name: keratin 19
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 44-50 kDa
• GenBank-Zugangsnummer: BC007628
• Gene symbol: KRT19
• Gene ID (NCBI): 3880
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.

Publikationen

Species	Application	Title
mouse	IHC,IF	Nat Commun; Interventional hydrogel microsphere vaccine as an immune amplifier for activated antitumour immunity after ablation therapy; Authors - Xiaoyu Liu
human	IHC	Sci Transl Med; PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.; Authors - Tian-Yi Jiang
human		Adv Sci (Weinh); PMID: 31728273; Authors - Qingming Tang
human		Nat Commun; Reversal of pancreatic desmoplasia by re-educating stellate cells with a tumour microenvironment-activated nanosystem.; Authors - Xuexiang Han
human	IHC	J Clin Invest; CCN1 induces hepatic ductular reaction through integrin αvβ5-mediated activation of NF-κB.; Authors - Ki-Hyun Kim
		Cell Host Microbe; Zika Virus Targets Different Primary Human Placental Cells, Suggesting Two Routes for Vertical Transmission.; Authors - Takako Tabata

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alessandro (Verified Customer) (07-27-2022)

No aspecific staining, great outcome

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: ROs

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