KRT33B Polyklonaler Antikörper

KRT33B Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 11048-1-AP

Synonyme

Keratin-33B, Keratin, type I cuticular Ha3-II, keratin 33B, K33B, HHA3-II

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  WB analysis of MCF-7 using 11048-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11048-1-AP (KRT33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11048-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11048-1-AP (KRT33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 11048-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 11048-1-AP (KRT33B antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 11048-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 11048-1-AP (KRT33B antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 11048-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11048-1-AP (KRT33B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11048-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11048-1-AP (KRT33B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11048-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11048-1-AP (KRT33B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11048-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11048-1-AP (KRT33B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 11048-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 11048-1-AP (KRT33B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

11048-1-AP bindet in WB, IHC, IF/ICC, ELISA KRT33B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KRT33B fusion protein Ag1522
• Vollständiger Name: keratin 33B
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC009971
• Gene symbol: KRT33B
• Gene ID (NCBI): 3884
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 33 belongs to type I keratin and is found in hair and nails. There are two isoforms of this protein, encoded by two separate genes, KRTHA3A and KRTHA3B.

Publikationen

Species	Application	Title
human	WB	Asian J Androl; PMID: 24994782; Authors - Yan-Min Ma