Cytokeratin 34 Polyklonaler Antikörper

Cytokeratin 34 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20449-1-AP

Synonyme

keratin 34, KRT34, keratin34, Keratin-34, KRT 34

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  WB analysis of HeLa using 20449-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 20449-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20449-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 20449-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 20449-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 20449-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin cancer using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung squamous cell carcinoma using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung squamous cell carcinoma using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin cancer using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin cancer using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 20449-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 20449-1-AP (KRT34 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A375-Zellen, A431-Zellen, HepG2-Zellen, L02-Zellen, Maushautgewebe
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe, human lung squamous cell carcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20449-1-AP bindet in WB, IHC, IF/ICC, ELISA Cytokeratin 34 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 34 fusion protein Ag14295
• Vollständiger Name: keratin 34
• Berechnetes Molekulargewicht: 436 aa, 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC041070
• Gene symbol: KRT34
• Gene ID (NCBI): 3885
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). KRT 34 is a type I keratin.