Cytokeratin 7 Polyklonaler Antikörper

Cytokeratin 7 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 22208-1-AP

Synonyme

Cell and organelle markers, CK 7, CK7, Cytokeratin 7, Cytoskeleton Marker, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7

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Galerie der Validierungsdaten

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  WB analysis of A431 using 22208-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22208-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 22208-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 22208-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 22208-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 22208-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 22208-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 22208-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 22208-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22208-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22208-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 22208-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 22208-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 22208-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 22208-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 22208-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 22208-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 22208-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22208-1-AP (Cytokeratin 7 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 22208-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22208-1-AP (Cytokeratin 7 antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 22208-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using Cytokeratin 7 antibody (22208-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 22208-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 7 antibody (22208-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 22208-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.20ug Anti-Human Cytokeratin 7 (22208-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Nierengewebe, humanes Ovarialkarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

22208-1-AP bindet in WB, IHC, IF, FC, ELISA Cytokeratin 7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 7 fusion protein Ag17528
• Vollständiger Name: keratin 7
• Berechnetes Molekulargewicht: 469 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC002700
• Gene symbol: KRT7
• Gene ID (NCBI): 3855
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Ginekol Pol; Expression profiles of human somatic mesenchymal stem cells derived from fresh endometrium, ectopic-endometrium and umbilical cord; Authors - Chunmei Li