Cytokeratin 7-specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Cytokeratin 7-specific Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(57)

Kat-Nr. : 17513-1-AP

Synonyme

Cytokeratin 7, KRT7, CK 7, CK7, CK-7

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  WB analysis using 17513-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 17513-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17513-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17513-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17513-1-AP (Cytokeratin 7-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 17513-1-AP

  IP result of anti-Cytokeratin 7-specific (IP:17513-1-AP, 4ug; Detection:17513-1-AP 1:1000) with HepG2 cells lysate 3600ug.

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  IHC staining of human lung cancer using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). heat mediated antigen retrieved with .

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  IHC staining of human breast cancer using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 40x lens). heat mediated antigen retrieved with .

• 

  IHC staining of human cervical cancer using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 17513-1-AP (KRT7-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 17513-1-AP (KRT7-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 17513-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Cytokeratin 7-specific antibody (17513-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 17513-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Cytokeratin 7-specific antibody (17513-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17513-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cytokeratin 7-specific antibody (17513-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 17513-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 7-specific antibody (17513-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 17513-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 7-specific antibody (17513-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 17513-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 7-specific antibody (17513-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 17513-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 7-specific (17513-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, Maus-Blasengewebe, Mauslungengewebe, Ratten-Blasengewebe, Rattenlungengewebe, T-47D-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Zervixkarzinomgewebe, humanes Nierengewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mauslebergewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:10000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 28 publications below
• IF: See 28 publications below

Produktinformation

17513-1-AP bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Cytokeratin 7-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: keratin 7
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: NM_005556
• Gene symbol: Cytokeratin 7
• Gene ID (NCBI): 3855
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. This antibody is specifically against KRT7.

Publikationen

Species	Application	Title
rat	IF	Transl Res; SERPINA5 may promote the development of preeclampsia by disruption of the uPA/uPAR pathway.; Authors - Yan Long
human	IHC	Clin Cancer Res; Integrative Genomic Analysis of Gemcitabine Resistance in Pancreatic Cancer by Patient-derived Xenograft Models.; Authors - Gang Yang
mouse	IHC	Angiogenesis; A novel lymphatic pattern promotes metastasis of cervical cancer in a hypoxic tumour-associated macrophage-dependent manner.; Authors - Xiao-Jing Chen
rat	IHC,IF	Proc Natl Acad Sci U S A; Up-regulation of CD81 inhibits cytotrophoblast invasion and mediates maternal endothelial cell dysfunction in preeclampsia.; Authors - Li Shen
		Cell Death Dis; METTL14 contributes to acute lung injury by stabilizing NLRP3 expression in an IGF2BP2-dependent manner; Authors - Fei Cao
mouse	IF	Cell Prolif; Insufficient GDF15 expression predisposes women to unexplained recurrent pregnancy loss by impairing extravillous trophoblast invasion; Authors - Chunzi Lyu