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Cytokeratin 77 Rekombinanter Antikörper

Cytokeratin 77 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240490A11

Kat-Nr. : 83415-3-PBS

Synonyme

KRT77, 240490A11, CK-1B, Cytokeratin,Keratin, Cytokeratin-1B

Filter:
  • Western Blot (WB) analysis of HeLa cells using Cytokeratin 77 Recombinant antibody (83415-3-RR)

    WB analysis of HeLa using 83415-3-RR (same clone as 83415-3-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 83415-3-RR (KRT77 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83415-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83415-3-RR

    Affinity and Kinetic Characterization of 83415-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83415-3-RR against Human KRT77 were performed. The affinity constant is 0.209 nM.

Geprüfte Anwendungen

Produktinformation

83415-3-PBS bindet in WB, ELISA Cytokeratin 77 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Cytokeratin 77 fusion protein Ag21942
Vollständiger Name keratin 77
Berechnetes Molekulargewicht 578 aa, 62 kDa
Beobachtetes Molekulargewicht61 kDa
GenBank-ZugangsnummerBC122558
Gene symbol KRT77
Gene ID (NCBI) 374454
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins.The KRT77 gene is a type II epithelial cell α-keratin gene family member that is expressed in the skin and eccrine sweat glands. KRT77 has N- and C-terminal domains and a central rod domain consisting of 4 alpha-helical regions separated by 3 nonhelical linker sequences.