Cytokeratin 8 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Cytokeratin 8 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 10384-1-AP

Synonyme

CARD2, Cell and organelle markers, CK 8, CK8, CYK8, Cytokeratin 8, Cytoskeleton Marker, K2C8, K8, keratin 8, KO, KRT8, Type II keratin Kb8

---

Galerie der Validierungsdaten

• 

  WB analysis using 10384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10384-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10384-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10384-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10384-1-AP

  IP Result of anti-Cytokeratin 8 (IP:10384-1-AP, 4ug; Detection:10384-1-AP 1:1000) with HeLa cells lysate 2000ug.

• 

  IHC staining of human tonsillitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10384-1-AP (Cytokeratin 8 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10384-1-AP (Cytokeratin 8 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10384-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 8 antibody (10384-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 10384-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 8 antibody (10384-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

• 

  IF Staining of human breast cancer using 10384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10384-1-AP

  Immunofluorescent analysis of HepG2 cells, using KRT8 antibody 10384-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HepG2 using 10384-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug Cytokeratin 8 antibody (10384-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

• 

  FC experiment of MCF-7 using 10384-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug Cytokeratin 8 antibody (10384-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

• 

  FC experiment of MCF-7 using 10384-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug Cytokeratin 8 antibody (10384-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, A549-Zellen, COLO 320-Zellen, HaCaT-Zellen, Maushautgewebe, MCF-7-Zellen, Rattenhautgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Appendizitis-Gewebe, humanes Kolongewebe, humanes Lebergewebe, humanes Mammakarzinomgewebe, humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Mammakarzinomgewebe
• Erfolgreiche Detektion in FC: HepG2-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:40000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Produktinformation

10384-1-AP bindet in WB, IP, IHC, IF, FC, ELISA Cytokeratin 8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, hund, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 8 fusion protein Ag0488
• Vollständiger Name: keratin 8
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC000654
• Gene symbol: KRT8
• Gene ID (NCBI): 3856
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.

Publikationen

Species	Application	Title
human	WB,IF,IP	J Cell Sci; SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network; Authors - Archan Chakraborty ; KD Validated
mouse	WB,IF	Front Cell Dev Biol; ESRRB Facilitates the Conversion of Trophoblast-Like Stem Cells From Induced Pluripotent Stem Cells by Directly Regulating CDX2.; Authors - Shuai Yu
human	WB,IF	J Cell Sci; SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network.; Authors - Archan Chakraborty ; KD Validated
human, goat, dog, pig	IHC	Anat Rec (Hoboken); Comparative study of the external auditory canal in humans and large mammals.; Authors - Jun Wang
human	WB,IF	Oncotarget; Alternative promotion and suppression of metastasis by JNK2 governed by its phosphorylation.; Authors - Sike Hu
mouse	IHC	Oncoscience; The helix-loop-helix transcriptional regulator Id4 is required for terminal differentiation of luminal epithelial cells in the prostate.; Authors - Dhanushka Hewa Bostanthirige

Verwandte Produkte

Cytokeratin 18 Polyclonal antibody

10830-1-AP

Cytokeratin 18 Monoclonal antibody

66187-1-Ig

Cytokeratin 19 Polyclonal antibody

10712-1-AP

Cytokeratin 19 Monoclonal antibody

60187-1-Ig

Vimentin Polyclonal antibody

10366-1-AP

ERK1/2 Polyclonal antibody

11257-1-AP

uPA/Urokinase Polyclonal antibody

17968-1-AP

Cytokeratin 7-specific Polyclonal antibody

17513-1-AP

Cytokeratin 14 Polyclonal antibody

10143-1-AP

TRAIL Polyclonal antibody

17235-1-AP