Cytokeratin 8 Polyklonaler Antikörper
Cytokeratin 8 Polyklonal Antikörper für WB, IHC, IF-P, FC (Intra), ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (2)
Anwendung
WB, IHC, IF-P, FC (Intra), ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 17514-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | A431-Zellen, A549-Zellen, C6-Zellen, HeLa-Zellen, MCF-7-Zellen |
Erfolgreiche Detektion in IHC | humanes Appendizitis-Gewebe, humanes Kolongewebe, humanes Prostatakarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF-P | Maushodengewebe |
Erfolgreiche Detektion in FC (Intra) | HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunhistochemie (IHC) | IHC : 1:2000-1:8000 |
Immunfluoreszenz (IF)-P | IF-P : 1:200-1:800 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 9 publications below |
IHC | See 12 publications below |
IF | See 10 publications below |
Produktinformation
17514-1-AP bindet in WB, IHC, IF-P, FC (Intra), ELISA Cytokeratin 8 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, hamster, Maus, Ratte, Zebrafisch |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Peptid |
Vollständiger Name | keratin 8 |
Berechnetes Molekulargewicht | 54 kDa |
Beobachtetes Molekulargewicht | 54 kDa |
GenBank-Zugangsnummer | NM_002273 |
Gene symbol | Cytokeratin 8 |
Gene ID (NCBI) | 3856 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo. This antibody is specifically against KRT8.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for Cytokeratin 8 antibody 17514-1-AP | Protokoll herunterladen |
IHC protocol for Cytokeratin 8 antibody 17514-1-AP | Protokoll herunterladenl |
IF protocol for Cytokeratin 8 antibody 17514-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Nat Aging Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer | ||
Oncogene The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer | ||
Clin Transl Med Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma. | ||
Sci Rep Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators. | ||
Sci Rep A method for the identification of lactate metabolism-related prognostic biomarkers and its validations in non-small cell lung cancer | ||
iScience Cysteine Dioxygenase Regulates the Epithelial Morphogenesis of Mammary Gland via Cysteine Sulfinic Acid. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Emma (Verified Customer) (11-29-2021) | Works very well by IF on FFPE tissue. Used at 1:50 with a Tris-EDTA antigen retrieval.
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FH Joleen (Verified Customer) (06-12-2019) | This antibody recognizes a band that matched with the expected molecular weight of KRT8.
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