Cytokeratin 8 Polyklonaler Antikörper

Cytokeratin 8 Polyklonal Antikörper für WB, IHC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(26)

Kat-Nr. : 17514-1-AP

Synonyme

KRT8, CARD2, CK 8, CK8, CK-8



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA431-Zellen, A549-Zellen, C6-Zellen, HeLa-Zellen, MCF-7-Zellen
Erfolgreiche Detektion in IHChumanes Appendizitis-Gewebe, humanes Kolongewebe, humanes Prostatakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PMaushodengewebe
Erfolgreiche Detektion in FC (Intra)HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:8000
Immunhistochemie (IHC)IHC : 1:2000-1:8000
Immunfluoreszenz (IF)-PIF-P : 1:200-1:800
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

17514-1-AP bindet in WB, IHC, IF-P, FC (Intra), ELISA Cytokeratin 8 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, hamster, Maus, Ratte, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name keratin 8
Berechnetes Molekulargewicht 54 kDa
Beobachtetes Molekulargewicht 54 kDa
GenBank-ZugangsnummerNM_002273
Gene symbol Cytokeratin 8
Gene ID (NCBI) 3856
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo. This antibody is specifically against KRT8.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Cytokeratin 8 antibody 17514-1-APProtokoll herunterladen
IHC protocol for Cytokeratin 8 antibody 17514-1-APProtokoll herunterladenl
IF protocol for Cytokeratin 8 antibody 17514-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouse,humanIF

Nat Aging

Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer

Authors - Yifei Cheng
humanWB

Oncogene

The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer

Authors - Mengjie Li
humanWB

Clin Transl Med

Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.

Authors - An-Dong Liu
mouseIHC

Sci Rep

Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators.

Authors - Shu Wei
humanIHC

Sci Rep

A method for the identification of lactate metabolism-related prognostic biomarkers and its validations in non-small cell lung cancer

Authors - Weiyang Yang
mouseWB,IF

iScience

Cysteine Dioxygenase Regulates the Epithelial Morphogenesis of Mammary Gland via Cysteine Sulfinic Acid.

Authors - Jianjun Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Emma (Verified Customer) (11-29-2021)

Works very well by IF on FFPE tissue. Used at 1:50 with a Tris-EDTA antigen retrieval.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Prostate
Cytokeratin 8 Antibody Immunofluorescence validation (1:50 dilution) in Prostate (Cat no:17514-1-AP)
FH

Joleen (Verified Customer) (06-12-2019)

This antibody recognizes a band that matched with the expected molecular weight of KRT8.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
Cytokeratin 8 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:17514-1-AP)