KRT81 Polyklonaler Antikörper

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• KD/KO Validated

KRT81 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11342-1-AP

Synonyme

ghHb1, ghHkb1, Hair keratin K2.9, Hb 1, HB1, hHAKB2 1, K81, keratin 81, Keratin, hair, basic, 1, Keratin, type II cuticular Hb1, KRT81, KRTHB1, MLN 137, MLN137, Type II hair keratin Hb1, Type II keratin Kb21

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Galerie der Validierungsdaten

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  WB analysis using 11342-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11342-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11342-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 11342-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 11342-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11342-1-AP

  IP Result of anti-KRT81 (IP:11342-1-AP, 4ug; Detection:11342-1-AP 1:300) with HEK-293 cells lysate 2800ug.

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  IHC staining of human colon cancer using 11342-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11342-1-AP (KRT81 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 11342-1-AP

  Immunofluorescent analysis of HepG2 cells, using KRT81 antibody 11342-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, humanes Milzgewebe, Maus-Pankreasgewebe, Maus-Thymusgewebe, MCF-7-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

11342-1-AP bindet in WB, IP, IHC, IF, ELISA KRT81 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KRT81 fusion protein Ag1890
• Vollständiger Name: keratin 81
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC021241
• Gene symbol: KRT81
• Gene ID (NCBI): 3887
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT81 is a type II keratin.

Publikationen

Species	Application	Title
human	IHC	Clin Cancer Res; A Preclinical Trial and Molecularly Annotated Patient Cohort Identify Predictive Biomarkers in Homologous Recombination-deficient Pancreatic Cancer.; Authors - Yifan Wang
		Oncol Rep; Hair keratin KRT81 is expressed in normal and breast cancer cells and contributes to their invasiveness.; Authors - Naoki Nanashima
human	WB	Heliyon; Single-cell data analysis of malignant epithelial cell heterogeneity in lung adenocarcinoma for patient classification and prognosis prediction; Authors - Xu Ran ; KD Validated
human,mouse	WB	Adv Sci (Weinh); Ectopic Expression of a Truncated Isoform of Hair Keratin 81 in Breast Cancer Alters Biophysical Characteristics to Promote Metastatic Propensity; Authors - Diane S Kang ; KD Validated