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KYNU Rekombinanter Antikörper

KYNU Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251437C8

Kat-Nr. : 86555-4-PBS

Synonyme

EC:3.7.1.3, Kynureninase, L kynurenine hydrolase, L-kynurenine hydrolase

Filter:
  • Western Blot (WB) analysis of various lysates using KYNU Recombinant antibody (86555-4-RR)

    WB analysis using 86555-4-RR (same clone as 86555-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86555-4-RR (KYNU antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86555-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using KYNU Recombinant antibody (86555-4-RR)

    IF Staining of A549 using 86555-4-RR (same clone as 86555-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using KYNU antibody (86555-4-RR, Clone: 251437C8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86555-4-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

86555-4-PBS bindet in WB, IF/ICC, Indirect ELISA KYNU und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen KYNU fusion protein Ag2365
Vollständiger Name kynureninase (L-kynurenine hydrolase)
Berechnetes Molekulargewicht465aa,52 kDa; 307aa,35 kDa
Beobachtetes Molekulargewicht45-52 kDa
GenBank-ZugangsnummerBC000879
Gene symbol KYNU
Gene ID (NCBI) 8942
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KYNU(kynureninase) is also named as L-kynurenine hydrolase and belongs to the kynureninase family. It is a pyridoxal-5'-phosphate-(pyridoxal-P)-depe11dent enzyme that catalyses the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively(PMID:8706755). In SDS-PAGE, it can detect a 48 kDa band lower than the preidicted molecular weight of 52 kDa due to limited proteolysis of the enzyme which might occur during handling of the protein(PMID:8706755).