Cytokeratin 77 Rekombinanter Antikörper

Cytokeratin 77 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240490A11

Kat-Nr. : 83415-3-RR

Synonyme

KRT77, 240490A11, CK-1B, Cytokeratin,Keratin, Cytokeratin-1B

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  WB analysis of HeLa using 83415-3-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 83415-3-RR (KRT77 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83415-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83415-3-RR against Human KRT77 were performed. The affinity constant is 0.209 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83415-3-RR bindet in WB, ELISA Cytokeratin 77 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Cytokeratin 77 fusion protein Ag21942
• Vollständiger Name: keratin 77
• Berechnetes Molekulargewicht: 578 aa, 62 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC122558
• Gene symbol: KRT77
• Gene ID (NCBI): 374454
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins.The KRT77 gene is a type II epithelial cell α-keratin gene family member that is expressed in the skin and eccrine sweat glands. KRT77 has N- and C-terminal domains and a central rod domain consisting of 4 alpha-helical regions separated by 3 nonhelical linker sequences.