Ki-67 Monoklonaler Antikörper

Ki-67 Monoklonal Antikörper für IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

1B9H2

Kat-Nr. : 66555-6-Ig

Synonyme

KI67, MKI67, 1B9H2, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67

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  IHC staining of human tonsillitis using 66555-6-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66555-6-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 66555-6-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 66555-6-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 66555-6-Ig

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 66555-6-Ig

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 66555-6-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Ki-67 antibody (66555-6-Ig, Clone: 1B9H2 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  IF Staining of HeLa using 66555-6-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Ki-67 antibody (66555-6-Ig, Clone: 1B9H2 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  FC experiment of A431 using 66555-6-Ig

  1x10^6 A431 cells were intracellularly stained with 0.2 μg Ki-67 Monoclonal antibody (66555-6-Ig, Clone:1B9H2,red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005), Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, human ovary cancer tissue, human rectal cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): A431-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:1000-1:5000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:1000-1:4000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.2 μg per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66555-6-Ig bindet in IHC, IF/ICC, FC (Intra), ELISA Ki-67 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Ki-67 fusion protein Ag26266
• Vollständiger Name: antigen identified by monoclonal antibody Ki-67
• Berechnetes Molekulargewicht: 359 kDa
• GenBank-Zugangsnummer: NM_002417
• Gene symbol: KI67
• Gene ID (NCBI): 4288
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.