Ki-67 Rekombinanter Antikörper

Ki-67 Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

241499E7

Kat-Nr. : 84192-4-RR

Synonyme

KI67, MKI67, 241499E7, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67

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  WB analysis using 84192-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84192-4-RR (Ki-67 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human Spleen using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded Human Spleen tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human Spleen using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded Human Spleen tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human renal cancer using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded Human renal cancer slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human renal cancer using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded Human renal cancer slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human Breast cancer using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded human Breast cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human Breast cancer using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded human Breast cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lung cancer using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1/CD54 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human lung cancer using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human thyroid cancer using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human thyroid cancer tissue using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human thyroid cancer using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human thyroid cancer tissue using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1/CD54 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red) (Cat No. PF00003).

• 

  IF Staining of HeLa using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of U2OS using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of A549 using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red) (Cat No. PF00003).

• 

  IF Staining of A431 using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red) (Cat No. PF00003).

• 

  IF Staining of HepG2 using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red) (Cat No. PF00003).

• 

  IF Staining of U2OS using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red) (Cat No. PF00003).

• 

  IF Staining of MCF-7 using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red) (Cat No. PF00003).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, MCF-7-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Mammakarzinomgewebe, humanes malignes Melanomgewebe, humanes Prostatakarzinomgewebe, humanes Hautkrebsgewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Lungenkarzinomgewebe, humanes Schilddrüsenkarzinomgewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, A431-Zellen, A549-Zellen, HepG2-Zellen, MCF-7-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

84192-4-RR bindet in WB, IHC, IF/ICC, IF-P, ELISA Ki-67 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: antigen identified by monoclonal antibody Ki-67
• Berechnetes Molekulargewicht: 359 kDa
• Beobachtetes Molekulargewicht: 359 kDa
• GenBank-Zugangsnummer: NM_002417
• Gene symbol: KI67
• Gene ID (NCBI): 4288
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

Publikationen

Species	Application	Title
human	IHC	Cell Biol Int; Hypoxic Cancer Cells-Derived Exosomes Strengthen the Development of Cancer Stem Cell-Like Properties Through Delivering LINC00665 in Thyroid Cancer Cells; Authors - Ming Zhou
human	IF	Int Immunopharmacol; ACNO hydrogel enhances diabetic wound healing by modulating the Bcl-2/Bax/Caspase-3/PARP pathway; Authors - Penghui Ye
human	IHC	Cancer Lett; Apigenin Inhibits Recurrent Bladder Cancer Progression by Targeting VEGF-β; Authors - Zhen-Duo Shi