Ki-67 Rekombinanter Antikörper

Ki-67 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

241768E2

Kat-Nr. : CL647-98224

Synonyme

Ki 67, Ki67, Mki67, 241768E2, Proliferation related Ki 67 antigen

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  FC experiment of C57BL/6 mouse splenocytes using CL647-98224

  1x10^6 untreated or Con A treated C57BL/6 mouse splenocytes were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse Ki-67 Rabbit RecAb (CL647-98224, Clone: 241768E2). Cells were co-stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of C57BL/6 mouse splenocytes using CL647-98224

  1x10^6 Con A treated C57BL/6 mouse splenocytes were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse Ki-67 Rabbit RecAb (CL647-98224, Clone: 241768E2) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). 1x10^6 untreated C57BL/6 mouse splenocytes were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse Ki-67 Rabbit RecAb (CL647-98224, Clone:241768E2)(black). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): Con A treated C57BL/6 mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-98224 bindet in FC (Intra) Ki-67 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Ki-67 fusion protein Eg1548
• Vollständiger Name: antigen identified by monoclonal antibody Ki 67
• Berechnetes Molekulargewicht: 351kDa
• GenBank-Zugangsnummer: NM_001081117.2
• Gene symbol: ki67
• Gene ID (NCBI): 17345
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki-67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.