Ki-67 Rekombinanter Antikörper

Ki-67 Rekombinant Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241768E2

Kat-Nr. : 84432-1-PBS

Synonyme

Mki67, Ki67, Ki 67, Proliferation related Ki 67 antigen, 241768E2

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  WB analysis of NIH/3T3 using 84432-1-RR (same clone as 84432-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84432-1-RR (Ki67/MKI67 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84432-1-RR (same clone as 84432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 84432-1-RR (same clone as 84432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 84432-1-RR (same clone as 84432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 84432-1-RR (same clone as 84432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 84432-1-RR (same clone as 84432-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Ki-67 antibody (84432-1-RR, Clone: 241768E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

• 

  IF Staining of C2C12 using 84432-1-RR (same clone as 84432-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Ki-67 antibody (84432-1-RR, Clone: 241768E2 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 84432-1-RR (same clone as 84432-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Ki-67 antibody (84432-1-RR, Clone: 241768E2 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01277-1

  Cytometric bead array standard curve of MP01277-1, MOUSE Ki-67 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84432-2-PBS. Detection antibody: 84432-1-PBS. Standard: Eg1548. Range: 0.625-40 ng/mL.

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Geprüfte Anwendungen

Produktinformation

84432-1-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Ki-67 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: antigen identified by monoclonal antibody Ki 67
• Berechnetes Molekulargewicht: 351kDa
• Beobachtetes Molekulargewicht: 350 kDa
• GenBank-Zugangsnummer: NM_001081117.2
• Gene symbol: ki67
• Gene ID (NCBI): 17345
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry.