Kininogen 1 Monoklonaler Antikörper

Kininogen 1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

10E8D11

Kat-Nr. : 66123-1-PBS

Synonyme

BDK, Fitzgerald factor, HMWK, Ile Ser Bradykinin Bradykinin, Kallidin I Lysyl bradykinin, kininogen 1, KNG, KNG1

---

• 

  WB analysis of human heart using 66123-1-Ig (same clone as 66123-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 66123-1-Ig (same clone as 66123-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human plasma using 66123-1-Ig (same clone as 66123-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66123-1-Ig (same clone as 66123-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66123-1-Ig (same clone as 66123-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66123-1-Ig (same clone as 66123-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66123-1-Ig (same clone as 66123-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66123-1-Ig (same clone as 66123-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Kininogen 1 antibody (66123-1-Ig, Clone: 10E8D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66123-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Kininogen 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Kininogen 1 fusion protein Ag19233
• Vollständiger Name: kininogen 1
• Berechnetes Molekulargewicht: 644 aa, 72 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC060039
• Gene symbol: Kininogen 1
• Gene ID (NCBI): 3827
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This gene is translated into High-molecular weight kininogen (HMWK) and low-molecular weight kininogen (LMWK) after alternative splicing. HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein.