• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

L-Plastin Rekombinanter Antikörper

L-Plastin Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251063A3

Kat-Nr. : 86299-3-PBS

Synonyme

LCP1, L PLASTIN, LCP 1, LCP-1, LPL

Filter:
  • Western Blot (WB) analysis of various lysates using L-Plastin Recombinant antibody (86299-3-RR)

    WB analysis using 86299-3-RR (same clone as 86299-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86299-3-RR (L-Plastin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86299-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using L-Plastin Recombinant antibody (86299-3-RR)

    IF Staining of Jurkat using 86299-3-RR (same clone as 86299-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using L-Plastin antibody (86299-3-RR, Clone: 251063A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86299-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86299-3-RR

    Affinity and Kinetic Characterization of 86299-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86299-3-RR against Human L-Plastin were performed. The affinity constant is 5.27 nM.

Geprüfte Anwendungen

Produktinformation

86299-3-PBS bindet in WB, IF/ICC, Indirect ELISA L-Plastin und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen L-Plastin fusion protein Ag3571
Vollständiger Name lymphocyte cytosolic protein 1 (L-plastin)
Berechnetes Molekulargewicht 627 aa, 64 kDa
Beobachtetes Molekulargewicht 64-70 kDa
GenBank-ZugangsnummerBC010271
Gene symbol L-Plastin
Gene ID (NCBI) 3936
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LCP1, also known as L-Plastin, is a member of the Plastin gene family. The family members include L-Plastin (LCP1), T-Plastin (PLS3), and I-Plastin (PLS1), which can regulate the dynamic reorganization of the cytoskeleton by binding and cross-linking actin filaments. In lymphocytes, LCP1 can regulate immune responses, especially during inflammation and infection. It acts as a key regulator of T-cell activation by responding to co-stimulatory signals through TCR/CD3 and CD2 or CD28, and regulates the cell-surface expression of IL2RA/CD25 and CD69 (PMID: 17294403).