L-Plastin Rekombinanter Antikörper

L-Plastin Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251063A3

Kat-Nr. : 86299-3-RR

Synonyme

LCP1, L PLASTIN, LCP 1, LCP-1, LPL

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  WB analysis using 86299-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86299-3-RR (L-Plastin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 86299-3-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using L-Plastin antibody (86299-3-RR, Clone: 251063A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86299-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86299-3-RR against Human L-Plastin were performed. The affinity constant is 5.27 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Jurkat-Zellen, Mausmilzgewebe, Ramos-Zellen
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86299-3-RR bindet in WB, IF/ICC, ELISA L-Plastin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: L-Plastin fusion protein Ag3571
• Vollständiger Name: lymphocyte cytosolic protein 1 (L-plastin)
• Berechnetes Molekulargewicht: 627 aa, 64 kDa
• Beobachtetes Molekulargewicht: 64-70 kDa
• GenBank-Zugangsnummer: BC010271
• Gene symbol: L-Plastin
• Gene ID (NCBI): 3936
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LCP1, also known as L-Plastin, is a member of the Plastin gene family. The family members include L-Plastin (LCP1), T-Plastin (PLS3), and I-Plastin (PLS1), which can regulate the dynamic reorganization of the cytoskeleton by binding and cross-linking actin filaments. In lymphocytes, LCP1 can regulate immune responses, especially during inflammation and infection. It acts as a key regulator of T-cell activation by responding to co-stimulatory signals through TCR/CD3 and CD2 or CD28, and regulates the cell-surface expression of IL2RA/CD25 and CD69 (PMID: 17294403).