L1CAM/CD171 Monoklonaler Antikörper

L1CAM/CD171 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

3H7B9

Kat-Nr. : 67115-1-Ig

Synonyme

L1CAM, 3H7B9, CAML1, CD171, HSAS

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  WB analysis using 67115-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67115-1-Ig (L1CAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig cerebellum using 67115-1-Ig

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67115-1-Ig (L1CAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 67115-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67115-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 67115-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 67115-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 67115-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67115-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67115-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 67115-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L1CAM antibody (67115-1-Ig, Clone: 3H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse brain using 67115-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L1CAM antibody (67115-1-Ig, Clone: 3H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, Maushirngewebe, Hausschwein-Cerebellum-Gewebe, Kaninchenhirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Hirngewebe, humanes Kolongewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

67115-1-Ig bindet in WB, IHC, IF-P, ELISA L1CAM/CD171 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: L1CAM/CD171 fusion protein Ag17706
• Vollständiger Name: L1 cell adhesion molecule
• Berechnetes Molekulargewicht: 1257 aa, 140 kDa
• Beobachtetes Molekulargewicht: 220 kDa
• GenBank-Zugangsnummer: BC126229
• Gene symbol: L1CAM
• Gene ID (NCBI): 3897
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).

Publikationen

Species	Application	Title
human	WB	J Neuroimmune Pharmacol; Brain-Derived Exosomal CircRNAs in Plasma Serve as Diagnostic Biomarkers for Acute Ischemic Stroke; Authors - Xinli Jiang
mouse	WB,IF	Cell Commun Signal; Extracellular vesicles from UTX-knockout endothelial cells boost neural stem cell differentiation in spinal cord injury; Authors - Yudong Liu
human	WB	J Extracell Vesicles; Isolation and quantification of L1CAM-positive extracellular vesicles on a chip as a potential biomarker for Parkinson's Disease; Authors - Danyu Li