L1CAM/CD171 Rekombinanter Antikörper

L1CAM/CD171 Rekombinant Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240978A7

Kat-Nr. : 84034-5-RR

Synonyme

L1CAM, 240978A7, CAML1, CD171, HSAS

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  IF Staining of SH-SY5Y using 84034-5-RR

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using L1CAM antibody (84034-5-RR, Clone: 240978A7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84034-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84034-5-RR against Human L1CAM were performed. The affinity constant is 0.522 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84034-5-RR bindet in IF/ICC, ELISA L1CAM/CD171 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: L1CAM/CD171 fusion protein Eg0209
• Vollständiger Name: L1 cell adhesion molecule
• Berechnetes Molekulargewicht: 1257 aa, 140 kDa
• GenBank-Zugangsnummer: BC126229
• Gene symbol: L1CAM
• Gene ID (NCBI): 3897
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).