L1CAM/CD171 Rekombinanter Antikörper

L1CAM/CD171 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250101C11

Kat-Nr. : 86305-2-RR

Synonyme

L1cam, N-CAM-L1, CAML1, CD171, N CAM L1

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  WB analysis using 86305-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86305-2-RR (L1CAM/CD171 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 86305-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 86305-2-RR (L1CAM/CD171 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 86305-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 86305-2-RR (L1CAM/CD171 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Neuro-2a using 86305-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using L1CAM/CD171 antibody (86305-2-RR, Clone: 250101C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Neuro-2a-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86305-2-RR bindet in WB, IHC, IF/ICC, ELISA L1CAM/CD171 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: L1CAM/CD171 fusion protein Eg3022
• Vollständiger Name: L1 cell adhesion molecule
• Berechnetes Molekulargewicht: 141 kDa
• Beobachtetes Molekulargewicht: 210-250 kDa
• GenBank-Zugangsnummer: N/A
• Gene symbol: L1cam
• Gene ID (NCBI): 16728
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310).