L1CAM/CD171 Rekombinanter Antikörper

L1CAM/CD171 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

240978A7

Kat-Nr. : 98130-1-PBS

Synonyme

L1CAM, 240978A7, CAML1, CD171, HSAS

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  FC experiment of SK-N-SH using 98130-1-RR (same clone as 98130-1-PBS)

  1x10^6 SK-N-SH cells were surface stained with 0.25 ug Anti-Human L1CAM/CD171 Rabbit Recombinant Antibody (98130-1-RR, Clone:240978A7) or 0.25 ug Isotype Control, and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed. This data was developed using the same antibody clone with 98130-1-PBS in a different storage buffer formulation.

• 

  FC experiment of SK-N-SH using 98130-1-RR (same clone as 98130-1-PBS)

  1x10^6 SK-N-SH cells were surface stained with 0.25 ug Anti-Human L1CAM/CD171 Rabbit Recombinant Antibody (98130-1-RR, Clone: 240978A7) (red) or Isotype Control (blue), and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed. This data was developed using the same antibody clone with 98130-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98130-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98130-1-RR against Human L1CAM/CD171 were performed. The affinity constant is 0.243 nM.

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Geprüfte Anwendungen

Produktinformation

98130-1-PBS bindet in FC L1CAM/CD171 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: L1CAM/CD171 fusion protein Eg0209
• Vollständiger Name: L1 cell adhesion molecule
• Berechnetes Molekulargewicht: 1257 aa, 140 kDa
• GenBank-Zugangsnummer: BC126229
• Gene symbol: L1CAM
• Gene ID (NCBI): 3897
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).