L1CAM/CD171 Rekombinanter Antikörper

L1CAM/CD171 Rekombinant Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250101C11

Kat-Nr. : 86305-2-PBS

Synonyme

L1cam, N-CAM-L1, CAML1, CD171, N CAM L1

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  WB analysis using 86305-2-RR (same clone as 86305-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86305-2-RR (L1CAM/CD171 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86305-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 86305-2-RR (same clone as 86305-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 86305-2-RR (L1CAM/CD171 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86305-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 86305-2-RR (same clone as 86305-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 86305-2-RR (L1CAM/CD171 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86305-2-PBS in a different storage buffer formulation.

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  IF Staining of Neuro-2a using 86305-2-RR (same clone as 86305-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using L1CAM/CD171 antibody (86305-2-RR, Clone: 250101C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86305-2-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02352-1

  The mean L1CAM concentration was determined to be 269.5 ng/mL in mouse serum.

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  Cytometric bead array standard curve of MP02352-1

  Cytometric bead array standard curve of MP02352-1, MOUSE L1CAM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86305-2-PBS. Detection antibody: 86305-3-PBS. Standard: Eg3022. Range: 0.781-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

86305-2-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA L1CAM/CD171 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: L1CAM/CD171 fusion protein Eg3022
• Vollständiger Name: L1 cell adhesion molecule
• Berechnetes Molekulargewicht: 141 kDa
• Beobachtetes Molekulargewicht: 210-250 kDa
• GenBank-Zugangsnummer: N/A
• Gene symbol: L1cam
• Gene ID (NCBI): 16728
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310).