L3MBTL3 Polyklonaler Antikörper

L3MBTL3 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28112-1-PBS

Synonyme

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  WB analysis using 28112-1-AP (same clone as 28112-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28112-1-AP (L3MBTL3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28112-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 28112-1-AP (same clone as 28112-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28112-1-AP (L3MBTL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28112-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 28112-1-AP (same clone as 28112-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28112-1-AP (L3MBTL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28112-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 28112-1-AP (same clone as 28112-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28112-1-AP (L3MBTL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28112-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 28112-1-AP (same clone as 28112-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28112-1-AP (L3MBTL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28112-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 28112-1-AP (same clone as 28112-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using L3MBTL3 antibody (28112-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28112-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28112-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA L3MBTL3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: L3MBTL3 fusion protein Ag27136
• Vollständiger Name: l(3)mbt-like 3 (Drosophila)
• Berechnetes Molekulargewicht: 88 aa, 3 kDa
• Beobachtetes Molekulargewicht: 88 kDa
• GenBank-Zugangsnummer: BC060845
• Gene symbol: L3MBTL3
• Gene ID (NCBI): 84456
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

L3MBTL3, also named as MBT1 and KIAA1798, belongs to the Malignant brain tumor (MBT) family. L3MBTL3 can bind to methyl lysine in histone, further affecting the transcription and its abnormal regulation associated with many diseases (PMID: 34373992). Additionally, researches have shown that L3MBTL3 plays a role in meduloblastoma formation and normal hematopoiesis in humans (PMID: 26317848). L3MBTL3 has 2 isoforms with the molecular mass of 86 and 88 kDa.