LAG-3/CD223 Polyklonaler Antikörper

LAG-3/CD223 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16616-1-PBS

Synonyme

CD223, LAG3, FDC, LAG 3, LAG-3

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  WB analysis using 16616-1-AP (same clone as 16616-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16616-1-AP (LAG-3/CD223 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 16616-1-AP (same clone as 16616-1-PBS)

  IP result of anti-LAG-3 (IP:16616-1-AP, 4ug; Detection:16616-1-AP 1:500) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16616-1-AP (LAG-3/CD223 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16616-1-AP (LAG-3/CD223 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LAG-3 antibody (16616-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LAG-3 antibody (16616-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16616-1-PBS bindet in WB, IHC, IF-P, IP, ELISA LAG-3/CD223 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LAG-3/CD223 fusion protein Ag9909
• Vollständiger Name: lymphocyte-activation gene 3
• Berechnetes Molekulargewicht: 525 aa, 57 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC052589
• Gene symbol: LAG-3
• Gene ID (NCBI): 3902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)