LAG-3/CD223 Rekombinanter Antikörper

LAG-3/CD223 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F14

Kat-Nr. : 80867-1-PBS

Synonyme

LAG-3, CD223, 1F14, LAG3, LAG 3

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  WB analysis of CTLL-2 using 80867-1-RR (same clone as 80867-1-PBS)

  CTLL-2 cells were subjected to SDS PAGE followed by western blot with 80867-1-RR (LAG-3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80867-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80867-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80867-1-RR against Human LAG-3/CD223 were performed. The affinity constant is 8.47 nM.

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Geprüfte Anwendungen

Produktinformation

80867-1-PBS bindet in WB, Indirect ELISA LAG-3/CD223 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: lymphocyte-activation gene 3
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: NM_002286
• Gene symbol: LAG-3
• Gene ID (NCBI): 3902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)