LAG-3/CD223 Rekombinanter Antikörper

LAG-3/CD223 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

241294E11

Kat-Nr. : CL594-98167

Synonyme

CD223, LAG-3, 241294E11, LAG 3, Lag3

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  FC experiment of mouse splenocytes using CL594-98167

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Human CD3 and 0.25 ug CoraLite®594 Anti-Mouse LAG-3/CD223 Rabbit RecAb (CL594-98167, Clone:241294E11). Cells were not fixed.

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  FC experiment of mouse splenocytes using CL594-98167

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug CoraLite®594 Anti-Mouse LAG-3/CD223 Rabbit RecAb (CL594-98167, Clone:241294E11) (red) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug CoraLite®594 Anti-Mouse LAG-3/CD223 Rabbit RecAb (CL594-98167, Clone: 241294E11) (black, dashed). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Anti-CD3/CD28 treated mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-98167 bindet in FC LAG-3/CD223 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: LAG-3/CD223 fusion protein Eg1220
• Vollständiger Name: lymphocyte-activation gene 3
• Berechnetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: NM_008479.2
• Gene symbol: CD223
• Gene ID (NCBI): 16768
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Lymphocyte activation gene 3 protein (LAG-3), also known as CD223, is a type I transmembrane protein belonging to the immunoglobulin superfamily (PMID: 1692078). It is a CD4-related molecule that binds MHC class II with high affinity (PMID: 1692078; 9159144). LAG-3 is expressed on activated T and NK cells. Besides, natural CD4(+)CD25(+) Tregs express LAG-3 upon activation (PMID: 15485628). LAG-3 negatively regulates T-cell function and homeostasis, and contributes to the suppressor activity of Tregs (PMID: 14644131; 15485628).