LAG-3/CD223 Rekombinanter Antikörper

LAG-3/CD223 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

243086D4

Kat-Nr. : FITC-98435-2

Synonyme

CD223, LAG3, FDC, LAG 3, LAG-3

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  FC experiment of human PBMCs using FITC-98435-2

  1x10^6 untreated or anti-CD3/CD28 and IL-2 treated human PBMCs were surface stained with CoraLite®594 Anti-Human CD8 and 5 ul FITC Plus Anti-Human LAG-3/CD223 Rabbit RecAb (FITC-98435-2, Clone: 243086D4). Cells were not fixed.

• 

  FC experiment of human PBMCs using FITC-98435-2

  1x10^6 anti-CD3/CD28 and IL-2 treated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human LAG-3/CD223 Rabbit RecAb (FITC-98435-2, Clone: 243086D4) (red) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human LAG-3/CD223 Rabbit RecAb (FITC-98435-2, Clone: 243086D4) (black, dashed). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Anti-CD3/CD28 and IL-2 treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-98435-2 bindet in FC LAG-3/CD223 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: LAG-3/CD223 fusion protein Eg31550
• Vollständiger Name: lymphocyte-activation gene 3
• Berechnetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: NM_002286
• Gene symbol: LAG-3
• Gene ID (NCBI): 3902
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)