LAG-3/CD223 Polyklonaler Antikörper

LAG-3/CD223 Polyklonal Antikörper für IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 29548-1-AP

Synonyme

LAG-3, CD223, lymphocyte activation gene 3, LAG3, LAG 3

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  IHC staining of human tonsillitis using 29548-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 29548-1-AP (LAG-3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 29548-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 29548-1-AP (LAG-3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 29548-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using LAG-3/CD223 antibody (29548-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 4 publications below

Produktinformation

29548-1-AP bindet in IHC, IF-P, ELISA LAG-3/CD223 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: lymphocyte-activation gene 3
• Berechnetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: NM_002286
• Gene symbol: LAG-3
• Gene ID (NCBI): 3902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)

Publikationen

Species	Application	Title
human	IHC	Front Immunol; Prognostic value and immune landscapes of TERT promoter methylation in triple negative breast cancer; Authors - Fei Lin
human	IHC	Eur J Med Res; A combined analysis of multi-omics data reveals the prognostic values and immunotherapy response of LAG3 in human cancers; Authors - Jinwu Peng
human	IHC	Clin Transl Immunology; Lymphocyte activation gene 3 served as a potential prognostic and immunological biomarker across various cancer types: a clinical and pan-cancer analysis; Authors - Yifan Liu
human	IHC	Cell Oncol (Dordr); Construction and validation of a regulatory T cells-based classification of renal cell carcinoma: an integrated bioinformatic analysis and clinical cohort study; Authors - Yuntao Yao