LAG-3/CD223 Rekombinanter Antikörper

LAG-3/CD223 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

1F14

Kat-Nr. : 80867-1-RR

Synonyme

LAG-3, CD223, 1F14, LAG3, LAG 3

---

• 

  WB analysis of CTLL-2 using 80867-1-RR

  CTLL-2 cells were subjected to SDS PAGE followed by western blot with 80867-1-RR (LAG-3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 80867-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80867-1-RR against Human LAG-3/CD223 were performed. The affinity constant is 8.47 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: CTLL-2 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 2 publications below

Produktinformation

80867-1-RR bindet in WB, IF, ELISA LAG-3/CD223 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: lymphocyte-activation gene 3
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: NM_002286
• Gene symbol: LAG-3
• Gene ID (NCBI): 3902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)

Publikationen

Species	Application	Title
human	WB	Cancer Sci; Screening of novel serum biomarkers for gastric cancer in coastal populations using a protein microarray; Authors - Yongdong Yi
mouse	IF	Cancer Lett; The deubiquitinase USP2a promotes tumor immunosuppression by stabilizing immune checkpoint B7-H4 in lung adenocarcinoma harboring EGFR-activating mutants; Authors - Youwei Lu
mouse	WB,IF	Neural Regen Res; Neuroprotective effects of lixisenatide against propagation of α-synuclein pathology in Parkinson's disease; Authors - Shangqi Sun
human	WB	Int Immunopharmacol; Targeting SPP1+ macrophages via the SPP1-CD44 axis reveals a key mechanism of immune suppression and tumor progression in ovarian cancer; Authors - Lisha Hou