LAIR1 Monoklonaler Antikörper

LAIR1 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1A5D1

Kat-Nr. : 67220-1-Ig

Synonyme

1A5D1, CD305, hLAIR1, LAIR 1, LAIR-1

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  WB analysis of THP-1 using 67220-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67220-1-Ig (LAIR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 67220-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67220-1-Ig (LAIR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of TF-1 using 67220-1-Ig

  TF-1 cells were subjected to SDS PAGE followed by western blot with 67220-1-Ig (LAIR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 67220-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67220-1-Ig (LAIR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 67220-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67220-1-Ig (LAIR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 67220-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LAIR1 antibody (67220-1-Ig, Clone: 1A5D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human tonsillitis using 67220-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LAIR1 antibody (67220-1-Ig, Clone: 1A5D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of THP-1 using 67220-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using LAIR1 antibody (67220-1-Ig, Clone: 1A5D1 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, HL-60-Zellen, TF-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe
• Erfolgreiche Detektion in IF/ICC: THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

67220-1-Ig bindet in WB, IHC, IF/ICC, IF-P, ELISA LAIR1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LAIR1 fusion protein Ag14459
• Vollständiger Name: leukocyte-associated immunoglobulin-like receptor 1
• Berechnetes Molekulargewicht: 287 aa, 31 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa
• GenBank-Zugangsnummer: BC027899
• Gene symbol: LAIR1
• Gene ID (NCBI): 3903
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Leukocyte-associated immunoglobulin-like receptor 1 (LAIR1) is a transmembrane glycoprotein with a single immunoglobulin-like domain and a cytoplasmic tail containing two immune receptor tyrosine-based inhibitory motifs (PMID: 9285412). LAIR1 is expressed on the majority of human PBMCs, including NK, T, B, monocytes, and dendritic cells, as well as the majority of thymocytes (PMID: 10229813). It functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of NK cells, B cells and T cells. LAIR1 is a collagen receptor (PMID: 16754721). Tumor-expressed collagens can modulate immune cell function through the inhibitory collagen receptor LAIR1 (PMID: 21955987).

Publikationen

Species	Application	Title
human	WB,IHC,IF	Cancer Med; Development and validation of a leukocyte-associated immunoglobulin-like receptor-1 prognostic signature for lower-grade gliomas.; Authors - Zhansheng Fang