LAIR1 Rekombinanter Antikörper

LAIR1 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242226B10

Kat-Nr. : 85010-1-RR

Synonyme

242226B10, CD305, LAIR-1, Leukocyte-associated immunoglobulin-like receptor 1, mLAIR1

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  WB analysis of mouse spleen using 85010-1-RR

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 85010-1-RR (LAIR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 85010-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85010-1-RR (LAIR1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 85010-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85010-1-RR (LAIR1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of RAW 264.7 using 85010-1-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using LAIR1 antibody (85010-1-RR, Clone: 242226B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 85010-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85010-1-RR against Mouse LAIR1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe
• Erfolgreiche Detektion in IHC: Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85010-1-RR bindet in WB, IHC, IF/ICC, ELISA LAIR1 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: LAIR1 fusion protein Eg1879
• Vollständiger Name: leukocyte-associated Ig-like receptor 1
• Berechnetes Molekulargewicht: 30kDa
• Beobachtetes Molekulargewicht: 35 kDa, 45 kDa
• GenBank-Zugangsnummer: NM_001113474.1
• Gene symbol: Lair1
• Gene ID (NCBI): 52855
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Leukocyte-associated immunoglobulin-like receptor 1 (LAIR1) is a transmembrane glycoprotein with a single immunoglobulin-like domain and a cytoplasmic tail containing two immune receptor tyrosine-based inhibitory motifs (PMID: 9285412). LAIR1 is expressed on the majority of human PBMCs, including NK, T, B, monocytes, and dendritic cells, as well as the majority of thymocytes (PMID: 10229813). It functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of NK cells, B cells and T cells. LAIR1 is a collagen receptor (PMID: 16754721). Tumor-expressed collagens can modulate immune cell function through the inhibitory collagen receptor LAIR1 (PMID: 21955987).