LAMA1 Polyklonaler Antikörper

LAMA1 Polyklonal Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF-P, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28697-1-AP

Synonyme

Laminin 1 subunit alpha, laminin, alpha 1, LAMA, Laminin subunit alpha-1, Laminin subunit alpha 1

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  WB analysis using 28697-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28697-1-AP (LAMA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of rat colon using 28697-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat colon tissue using LAMA1 antibody (28697-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IF-P: Ratten-Kolongewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

28697-1-AP bindet in WB, IF-P, ELISA LAMA1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LAMA1 fusion protein Ag29329
• Vollständiger Name: laminin, alpha 1
• Berechnetes Molekulargewicht: 337 kDa
• Beobachtetes Molekulargewicht: 337 kDa
• GenBank-Zugangsnummer: NM_005559
• Gene symbol: LAMA1
• Gene ID (NCBI): 284217
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LAMA1 is also known as laminin subunit α1. Laminins are a family of glycoproteins found in the extracellular matrix that comprises the basement membrane. Laminins have a heterotrimeric structure composed of an α, β, and γ chain. Numerous biological processes are known to be directed by them, including cell adhesion, mitogenesis, differentiation, and metastasis, all of which contribute to carcinogenesis. Tissue distribution of LAMA1 occurred mainly in early epithelial development and some adult epithelia. Recent reports have shown that laminin-1 acts as an efficient attachment protein for a large variety of cultured cell types in vitro. (PMID: 36824663)