Laminin beta 1 Monoklonaler Antikörper

Laminin beta 1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

1B8E9

Kat-Nr. : 67705-1-Ig

Synonyme

CLM, LAMB1, Laminin 1 subunit beta, Laminin 10 subunit beta, Laminin 12 subunit beta, Laminin 2 subunit beta, Laminin 6 subunit beta, Laminin 8 subunit beta, Laminin B1 chain, Laminin beta 1, Laminin subunit beta 1, laminin, beta 1

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  WB analysis using 67705-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67705-1-Ig (LAMB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67705-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67705-1-Ig (LAMB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, NIH/3T3-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67705-1-Ig bindet in WB, ELISA Laminin beta 1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Laminin beta 1 fusion protein Ag19182
• Vollständiger Name: laminin, beta 1
• Berechnetes Molekulargewicht: 1786 aa, 198 kDa
• Beobachtetes Molekulargewicht: 220-240 kDa
• GenBank-Zugangsnummer: BC113455
• Gene symbol: Laminin beta 1
• Gene ID (NCBI): 3912
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Laminins are a family of basement membrane glycoproteins composed of three nonidentical chains, laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) (PMID: 7959779). Laminins contribute to the structure of extracellular matrix and influence the behavior of associated cells, such as adhesion, differentiation, migration, phenotype stability, and resistance to anoikis (PMID: 23057746). Laminin beta 1 (LAMB1) is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin-1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. Laminin beta 1 is also a subunit of laminin-2 (laminin-211 or merosin), laminin-6 (laminin-311 or K-laminin), laminin-8 (laminin-411), laminin-10 (laminin-511) and laminin-12 (laminin-213).

Publikationen

Species	Application	Title
human	WB	Cell Rep; DOT1L/H3K79me2 represses HIV-1 reactivation via recruiting DCAF1; Authors - Fenfei Liang
		Diabet Med; Mouse islet-derived stellate cells are similar to, but distinct from, mesenchymal stromal cells and influence the beta cell function; Authors - Wei Xu