LAMB2 Polyklonaler Antikörper

LAMB2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 30943-1-AP

Synonyme

S LAM beta, LAMS, Laminin 3 subunit beta, Laminin 15 subunit beta, Laminin 14 subunit beta

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  WB analysis using 30943-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30943-1-AP (LAMB2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30943-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30943-1-AP (LAMB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30943-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 30943-1-AP (LAMB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 30943-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30943-1-AP (LAMB2 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Mausherzgewebe, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30943-1-AP bindet in WB, IHC, ELISA LAMB2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LAMB2 fusion protein Ag33468
• Vollständiger Name: laminin, beta 2 (laminin S)
• Berechnetes Molekulargewicht: 196 kDa
• Beobachtetes Molekulargewicht: 195-200 kDa
• GenBank-Zugangsnummer: NM_002292.3
• Gene symbol: LAMB2
• Gene ID (NCBI): 3913
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LAMB2 (laminin subunit beta 2), also known as LAMS. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. This gene encodes the beta chain isoform laminin, beta 2. It is enriched in the basement membrane of muscles at the neuromuscular junctions, kidney glomerulus and vascular smooth muscle. Transgenic mice in which the beta 2 chain gene was inactivated by homologous recombination, showed defects in the maturation of neuromuscular junctions and impairment of glomerular filtration. The calculated molecular weight of LAMB2 is 200 kDa.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aditya (Verified Customer) (06-16-2025)

works well, clean bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000