LAMB2 Polyklonaler Antikörper

LAMB2 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30943-1-PBS

Synonyme

S LAM beta, LAMS, Laminin 3 subunit beta, Laminin 15 subunit beta, Laminin 14 subunit beta

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  WB analysis using 30943-1-AP (same clone as 30943-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30943-1-AP (LAMB2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30943-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 30943-1-AP (same clone as 30943-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30943-1-AP (LAMB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30943-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 30943-1-AP (same clone as 30943-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 30943-1-AP (LAMB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30943-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 30943-1-AP (same clone as 30943-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30943-1-AP (LAMB2 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30943-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

30943-1-PBS bindet in WB, IHC, Indirect ELISA LAMB2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LAMB2 fusion protein Ag33468
• Vollständiger Name: laminin, beta 2 (laminin S)
• Berechnetes Molekulargewicht: 196 kDa
• Beobachtetes Molekulargewicht: 195-200 kDa
• GenBank-Zugangsnummer: NM_002292.3
• Gene symbol: LAMB2
• Gene ID (NCBI): 3913
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LAMB2 (laminin subunit beta 2), also known as LAMS. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. This gene encodes the beta chain isoform laminin, beta 2. It is enriched in the basement membrane of muscles at the neuromuscular junctions, kidney glomerulus and vascular smooth muscle. Transgenic mice in which the beta 2 chain gene was inactivated by homologous recombination, showed defects in the maturation of neuromuscular junctions and impairment of glomerular filtration. The calculated molecular weight of LAMB2 is 200 kDa.