• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

LAMB3 Rekombinanter Antikörper

LAMB3 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242442A8

Kat-Nr. : 85055-4-PBS

Synonyme

Laminin B1k chain, 242442A8, Epiligrin subunit bata, Kalinin B1 chain, Kalinin subunit beta

Filter:
  • Western Blot (WB) analysis of various lysates using LAMB3 Recombinant antibody (85055-4-RR)

    WB analysis using 85055-4-RR (same clone as 85055-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85055-4-RR (LAMB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85055-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using LAMB3 Recombinant antibody (85055-4-RR)

    IHC staining of human colon using 85055-4-RR (same clone as 85055-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85055-4-RR (LAMB3 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85055-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85055-4-RR

    Affinity and Kinetic Characterization of 85055-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85055-4-RR against Human LAMB3 were performed. The affinity constant is 0.244 nM.

Geprüfte Anwendungen

Produktinformation

85055-4-PBS bindet in WB, IHC, Indirect ELISA LAMB3 und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen LAMB3 fusion protein Ag25192
Vollständiger Name laminin, beta 3
Berechnetes Molekulargewicht 1172 aa, 130 kDa
Beobachtetes Molekulargewicht130-140 kDa
GenBank-ZugangsnummerBC075838
Gene symbol LAMB3
Gene ID (NCBI) 3914
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LAMB3 (β3 subunit of laminin) is an important extracellular matrix protein, which plays an important role in cell adhesion, migration, signal transduction and the formation and stability of basement membrane. LAMB3 plays a key role in the basement membrane of skin and mucosa, maintaining the adhesion between epidermis and dermis. Its abnormal function will lead to skin bullous disease (such as severe borderline epidermolaxis, sJEB), which is characterized by blisters and ulcers on skin and mucosa. LAMB3 is highly expressed in many cancers, including pancreatic ductal adenocarcinoma (PDAC), lung cancer, head and neck squamous cell carcinoma, etc., which is closely related to the proliferation, invasion and metastasis of tumor cells.