CD107b / LAMP2 Polyklonaler Antikörper

CD107b / LAMP2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(69)

Kat-Nr. : 27823-1-AP

Synonyme

CD107b, Cell and organelle markers, LAMP 2, LAMP2, LAMPB, LGP110, Lysosome Marker

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  WB analysis using 27823-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27823-1-AP (LAMP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 27823-1-AP

  pig liver tissue were subjected to SDS PAGE followed by western blot with 27823-1-AP (LAMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 27823-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27823-1-AP (LAMP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 27823-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27823-1-AP (LAMP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 27823-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27823-1-AP (LAMP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 27823-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27823-1-AP (LAMP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 27823-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27823-1-AP (LAMP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Plazenta-Gewebe, Jurkat-Zellen, Hausschwein-Lebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 49 publications below
• IHC: See 3 publications below
• IF: See 23 publications below

Produktinformation

27823-1-AP bindet in WB, IHC, IF, ELISA CD107b / LAMP2 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD107b / LAMP2 fusion protein Ag27183
• Vollständiger Name: lysosomal-associated membrane protein 2
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC002965
• Gene symbol: LAMP2
• Gene ID (NCBI): 3920
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis. It may also functions in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides.

Publikationen

Species	Application	Title
human	WB	Nature; 7-Dehydrocholesterol dictates ferroptosis sensitivity; Authors - Yaxu Li
human	WB	J Extracell Vesicles; Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.; Authors - Xiaoyu Yu
	WB	Autophagy; USP19 (ubiquitin specific peptidase 19) promotes TBK1 (TANK-binding kinase 1) degradation via chaperone-mediated autophagy.; Authors - Xibao Zhao
pig	IF	Autophagy; Extracellular vesicles originating from autophagy mediate an antibody-resistant spread of classical swine fever virus in cell culture; Authors - Tao Wang
human	WB	Oxid Med Cell Longev; Restoration of Autophagic Flux Rescues Oxidative Damage and Mitochondrial Dysfunction to Protect against Intervertebral Disc Degeneration.; Authors - Liang Kang
pig	WB	J Anim Sci Biotechnol; Glyphosate exposure deteriorates oocyte meiotic maturation via induction of organelle dysfunctions in pigs.; Authors - Chunhua Xing