LAMTOR1 Polyklonaler Antikörper

LAMTOR1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29611-1-AP

Synonyme

C11orf59, p27RF-Rho, p27RF Rho, p27Kip1-releasing factor from RhoA, Lipid raft adaptor protein p18

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  WB analysis using 29611-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29611-1-AP (LAMTOR1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IF Staining of U-87 MG using 29611-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using LAMTOR1 antibody (29611-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: U-87 MG-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

29611-1-AP bindet in WB, IF/ICC, ELISA LAMTOR1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LAMTOR1 fusion protein Ag31215
• Vollständiger Name: chromosome 11 open reading frame 59
• Berechnetes Molekulargewicht: 161 aa, 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC001706
• Gene symbol: LAMTOR1
• Gene ID (NCBI): 55004
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LAMTOR1 (Late Endosome/Lysosome-Associated Membrane Protein 1) is a membrane protein specifically localized to the surface of late endosomes/lysosomes and is involved in lysosomal transport and maturation. LAMTOR1 is a key component of the lysosomal articulating protein complex Ragulator, which is required for the localization of the Ragulator complex to lysosomes. LAMTOR1 is a key component of the lysosomal bridging protein complex Ragulator and is required for the localization of the Ragulator complex to the lysosome. LAMTOR1 is involved in autophagy, cell growth, immune regulation, cancer development, and regulatory pathways of glucose metabolism. Recent studies have shown that in calorie restriction, lithocholic acid activates AMPK through the glucose-sensing pathway ( the lysosomal pathway ) and the body senses the metabolic signals induced by calorie restriction and exerts its role in slowing down the effects of aging and prolonging the lifespan, whereas deletion of LAMTOR1 results in the inability of AMPK to be activated in order to play its role.