LAP3 Monoklonaler Antikörper

LAP3 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B11B8

Kat-Nr. : 66417-1-PBS

Synonyme

Cytosol aminopeptidase, LAP, LAP 3, LAP3, LAPEP, leucine aminopeptidase 3, Leucyl aminopeptidase, PEPS, Peptidase S, Proline aminopeptidase, Prolyl aminopeptidase

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  WB analysis of NIH/3T3 using 66417-1-Ig (same clone as 66417-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66417-1-PBS in a different storage buffer formulation.

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  WB analysis of C2C12 using 66417-1-Ig (same clone as 66417-1-PBS)

  C2C12 cell were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66417-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66417-1-Ig (same clone as 66417-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66417-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66417-1-Ig (same clone as 66417-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66417-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 66417-1-Ig (same clone as 66417-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66417-1-Ig (LAP3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66417-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 66417-1-Ig (same clone as 66417-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66417-1-Ig (LAP3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66417-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66417-1-Ig (same clone as 66417-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LAP3 antibody (66417-1-Ig, Clone: 1B11B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66417-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66417-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA LAP3 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LAP3 fusion protein Ag6300
• Vollständiger Name: leucine aminopeptidase 3
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC065564
• Gene symbol: LAP3
• Gene ID (NCBI): 51056
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LAP3(Leucine aminopeptidase 3) is also named as LAPEP, PEPS(peptidase S) and belongs to the peptidase M17 family. It can catalyzes the removal of unsubstituted N-terminal amino-acids from various peptides and be presumably involved in the processing end regular turnover of intracellular proteins. PEPS is found in a wide variety of tissues and cultured cells but not in red cells and skin. The enzyme can utilize several di-, tri-, and tetrapeptides as substrates and is the slowest migrating of the peptidases after electrophoresis.(PMID:689684). LAP3 has two isoform with MW of 52 and 56 kDa.