LARG Polyklonaler Antikörper

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LARG Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22441-1-PBS

Synonyme

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  WB analysis of Jurkat using 22441-1-AP (same clone as 22441-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22441-1-AP (LARG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 22441-1-AP (same clone as 22441-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 22441-1-AP (LARG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 22441-1-AP (same clone as 22441-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22441-1-AP (LARG Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 22441-1-AP (same clone as 22441-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22441-1-AP (LARG Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 22441-1-AP (same clone as 22441-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using ARHGEF12 antibody 22441-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 22441-1-AP (same clone as 22441-1-PBS)

  Immunofluorescent analysis of HeLa cells using 22441-1-AP (LARG antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

22441-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA LARG und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LARG fusion protein Ag18211
• Vollständiger Name: Rho guanine nucleotide exchange factor (GEF) 12
• Berechnetes Molekulargewicht: 1544 aa, 173 kDa
• Beobachtetes Molekulargewicht: 220 kDa
• GenBank-Zugangsnummer: BC063117
• Gene symbol: LARG
• Gene ID (NCBI): 23365
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LARG, also known as ARHGEF12, is one of three regulator of G protein signalling (RGS) domain-containing RhoGEFs. LARG activates the ROCK pathway downstream of Gα12/13 signaling, leading to cytoskeletal reorganisation. LARG performs this activity either as a homodimer or as a heterodimer with Arhgef11. LARG has been implicated in many diseases, including cancer, heart disease, and in HIV viral replication. LARG is phosphorylated and dephosphorylated during mitosis and that Cdk1 is the mitotic kinase responsible for LARG phosphorylation. We got 220 kDa in western blotting, maybe due to its phosphorylation.