LARP4 Polyklonaler Antikörper

LARP4 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 16529-1-AP

Synonyme

La related protein 4, LARP4, PP13296

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  WB analysis of Y79 using 16529-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 16529-1-AP (LARP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 16529-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 16529-1-AP (LARP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 16529-1-AP

  IP result of anti-LARP4 (IP:16529-1-AP, 4ug; Detection:16529-1-AP 1:300) with Y79 cells lysate 2000ug.

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  IHC staining of human brain using 16529-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 16529-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 16529-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 16529-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16529-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16529-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 16529-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 16529-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16529-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16529-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 16529-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16529-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen
• Erfolgreiche IP: Y79-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Nierengewebe, humanes Lungengewebe, humanes Eierstockgewebe, humanes Milzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Produktinformation

16529-1-AP bindet in WB, IHC, IF, IP, ELISA LARP4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LARP4 fusion protein Ag9777
• Vollständiger Name: La ribonucleoprotein domain family, member 4
• Berechnetes Molekulargewicht: 188 aa, 21 kDa, 81 kDa
• Beobachtetes Molekulargewicht: 81 kDa
• GenBank-Zugangsnummer: BC022377
• Gene symbol: LARP4
• Gene ID (NCBI): 113251
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	WB,IF,IP	Front Cell Dev Biol; Interaction of LARP4 to filamin A mechanosensing domain regulates cell migrations; Authors - Zhenfeng Mao
human	IP	Nucleic Acids Res; SHIFTR enables the unbiased identification of proteins bound to specific RNA regions in live cells; Authors - Jens Aydin
human	WB,IF	Vet Microbiol; Cellular RNA-binding proteins LARP4 and PABPC1 synergistically facilitate viral translation of coronavirus PEDV; Authors - Jing Wang ; KD Validated