LASP1 Monoklonaler Antikörper

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• KD/KO Validated

LASP1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1G4B6

Kat-Nr. : 68080-1-PBS

Synonyme

1G4B6, LASP-1, LIM and SH3 protein 1, MLN50

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  WB analysis using 68080-1-Ig (same clone as 68080-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68080-1-Ig (LASP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68080-1-Ig (same clone as 68080-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68080-1-Ig (LASP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human peripheral blood platelets using 68080-1-Ig (same clone as 68080-1-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 68080-1-Ig (LASP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68080-1-Ig (same clone as 68080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68080-1-Ig (same clone as 68080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 68080-1-Ig (same clone as 68080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 68080-1-Ig (same clone as 68080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 68080-1-Ig (same clone as 68080-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using LASP1 antibody (68080-1-Ig, Clone: 1G4B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68080-1-PBS bindet in WB, IHC, IF/ICC, ELISA LASP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LASP1 fusion protein Ag18101
• Vollständiger Name: LIM and SH3 protein 1
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 35-38 kDa
• GenBank-Zugangsnummer: BC012460
• Gene symbol: LASP1
• Gene ID (NCBI): 3927
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LASP1(LIM and SH3 protein 1), also known as MLN50, is a 261 amino acid protein that localizes to both the cytoplasm and the cytoskeleton(PMID: 7589475). LASP1 consists of an N-terminal LIM-domain with two zinc finger motifs, followed by two central actin-binding nebulin repeats, flanked by a linker region and a C-terminal SH3 domain (PMID: 17177073, 9848085). LASP-1 interacts with F-Actin and plays an important role in the regulation of Actin-associated cytoskeletal organization. Agonist-dependent changes in LASP1 phosphorylation may regulate Actin-related ion transport activities in epithelial cells (PMID: 15465019,12571245). Overexpression of LASP-1 is associated with breast cancer, and plays a role in tumor transformation and metastasis (PMID: 17956604).