LASS2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

LASS2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 20344-1-AP

Synonyme

CerS2, L3, LASS2, SP260, TMSG1

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Galerie der Validierungsdaten

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  WB analysis using 20344-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20344-1-AP (LASS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20344-1-AP

  WB result of LASS2 antibody (20344-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-LASS2 transfected HeLa cells.

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  WB analysis using 20344-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20344-1-AP (LASS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20344-1-AP

  IP Result of anti-LASS2 (IP:20344-1-AP, 4ug; Detection:20344-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human liver using 20344-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20344-1-AP (LASS2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 20344-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20344-1-AP (LASS2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse lung using 20344-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20344-1-AP (LASS2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse lung using 20344-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20344-1-AP (LASS2 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HeLa-Zellen, HepG2-Zellen, Rattenlebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

20344-1-AP bindet in WB, IP, IHC, IF, ELISA LASS2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LASS2 fusion protein Ag14151
• Vollständiger Name: LAG1 homolog, ceramide synthase 2
• Berechnetes Molekulargewicht: 380 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa, 37 kDa
• GenBank-Zugangsnummer: BC010032
• Gene symbol: LASS2
• Gene ID (NCBI): 29956
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LASS2, also known as TMSG1, is a novel suppressor of human cancer metastasis. As one member of LASS family, including LASS1-6, LASS2 mRNA is at the highest level of all LASS members, and has the broadest tissue distribution, particularly abundant in the liver, kidney and brain in mice. The biological roles of LASS2 include protection from aging , hepatic INS resistance, and hepatocellular carcinoma (HCC) progression. LASS2 has been correlated with the degree of invasion and recurrence of carcinomas of the prostate, liver, breast and bladder.

Publikationen

Species	Application	Title
mouse	WB	Cardiovasc Res; Nogo-A reduces ceramide de novo biosynthesis to protect from heart failure.; Authors - Linda Sasset ; KD Validated
human	WB,IHC,IF	J Cancer; LASS2 impairs proliferation of glioma stem cells and migration and invasion of glioma cells mainly via inhibition of EMT and apoptosis promotion.; Authors - Wei-Jiang Zhao ; KD Validated
human	WB	Exp Cell Res; MicroRNA-98 promotes drug resistance and regulates mitochondrial dynamics by targeting LASS2 in bladder cancer cells.; Authors - Ting Luan ; KD Validated
human	WB,IF	J Cancer; LASS2 regulates invasion and chemoresistance via ERK/Drp1 modulated mitochondrial dynamics in bladder cancer cells.; Authors - Lijuan Huang ; KD Validated
human	WB	Tumour Biol; miR-9 promotes cell proliferation and inhibits apoptosis by targeting LASS2 in bladder cancer.; Authors - Haifeng Wang