LASS2 Rekombinanter Antikörper

LASS2 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241898E11

Kat-Nr. : 84514-4-RR

Synonyme

241898E11, Ceramide synthase 2, CerS2, EC:2.3.1.24, EC:2.3.1.297

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  WB analysis using 84514-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84514-4-RR (LASS2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of Human Hepatocellular cancer using 84514-4-RR

  Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84514-4-RR (LASS2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human Hepatocellular cancer using 84514-4-RR

  Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84514-4-RR (LASS2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84514-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84514-4-RR against Human LASS2 were performed. The affinity constant is 0.549 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, HepG2-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Human Hepatocellular cancer; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84514-4-RR bindet in WB, IHC, ELISA LASS2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: LASS2 fusion protein Ag14151
• Vollständiger Name: LAG1 homolog, ceramide synthase 2
• Berechnetes Molekulargewicht: 380 aa, 45 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC010032
• Gene symbol: LASS2
• Gene ID (NCBI): 29956
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LASS2, also known as TMSG1, is a novel suppressor of human cancer metastasis. As one member of LASS family, including LASS1-6, LASS2 mRNA is at the highest level of all LASS members, and has the broadest tissue distribution, particularly abundant in the liver, kidney and brain in mice. The biological roles of LASS2 include protection from aging , hepatic INS resistance, and hepatocellular carcinoma (HCC) progression. LASS2 has been correlated with the degree of invasion and recurrence of carcinomas of the prostate, liver, breast and bladder.