LATS1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

LATS1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17049-1-PBS

Synonyme

WARTS, Serine/threonine-protein kinase LATS1, LATS 1, h-warts, EC:2.7.11.1

---

• 

  WB analysis of HepG2 using 17049-1-AP (same clone as 17049-1-PBS)

  WB result of LATS1 antibody (17049-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LATS1 transfected HepG2 cells. This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 17049-1-AP (same clone as 17049-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17049-1-AP (LATS1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 17049-1-AP (same clone as 17049-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17049-1-AP (LATS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 17049-1-AP (same clone as 17049-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 17049-1-AP (LATS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 17049-1-AP (same clone as 17049-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17049-1-AP (LATS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 17049-1-AP (same clone as 17049-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17049-1-AP (LATS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 17049-1-AP (same clone as 17049-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17049-1-AP (LATS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 17049-1-AP (same clone as 17049-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17049-1-AP (LATS1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 17049-1-AP (same clone as 17049-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17049-1-AP (LATS1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 17049-1-AP (same clone as 17049-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LATS1 antibody (17049-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 17049-1-AP (same clone as 17049-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LATS1 antibody (17049-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

17049-1-PBS bindet in WB, IHC, IF/ICC, ELISA LATS1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LATS1 fusion protein Ag10709
• Vollständiger Name: LATS, large tumor suppressor, homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 15 kDa, 127 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa, 120 kDa
• GenBank-Zugangsnummer: BC015665
• Gene symbol: LATS1
• Gene ID (NCBI): 9113
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LATS1(Large tumor suppressor homolog 1) is also named as WARTS and belongs to the AGC Ser/Thr protein kinase family. The gene encodes a highly conserved (from fly to human) protein kinase that plays a crucial role in the prevention of tumor formation by controlling the progression of mitosis. The expression of both long(170 kDa) and short lats1 isoforms(120 kDa) in vertebrate retinal cells raises the possibility that these lats1 proteins may act as negative key regulators of the cell cycle each of them performing a unique role (PMID:15777619). In mammalian cells, LATS1 was phosphorylated in a cell cycle-dependent manner and complexed with CDC2 in early mitosis (PMID:9988268). LATS1 also can be detected as 120 kDa and 140-150 kDa, and play a key role in the regulation of Hippo pathway (PMID: 27940445).