LATS1 Monoklonaler Antikörper

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• KD/KO Validated

LATS1 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A8F1

Kat-Nr. : 66569-1-PBS

Synonyme

2A8F1, EC:2.7.11.1, h-warts, LATS 1, Serine/threonine-protein kinase LATS1

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  WB analysis using 66569-1-Ig (same clone as 66569-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66569-1-Ig (LATS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66569-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66569-1-Ig (same clone as 66569-1-PBS)

  WB result of LATS1 antibody (66569-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-LATS1 transfected HepG2 cells. This data was developed using the same antibody clone with 66569-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66569-1-Ig (same clone as 66569-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66569-1-Ig (LATS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66569-1-PBS in a different storage buffer formulation.

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  WB analysis of T-47D using 66569-1-Ig (same clone as 66569-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 66569-1-Ig (LATS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66569-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66569-1-Ig (same clone as 66569-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66569-1-Ig (LATS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66569-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66569-1-Ig (same clone as 66569-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66569-1-Ig (LATS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66569-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66569-1-PBS bindet in WB, Indirect ELISA LATS1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LATS1 fusion protein Ag11140
• Vollständiger Name: LATS, large tumor suppressor, homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 15 kDa, 127 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa, 120 kDa
• GenBank-Zugangsnummer: BC015665
• Gene symbol: LATS1
• Gene ID (NCBI): 9113
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LATS1(Large tumor suppressor homolog 1) is also named as WARTS and belongds to the AGC Ser/Thr protein kinase family. The gene encodes a highly conserved (from fly to human) protein kinase that plays a crucial role in the prevention of tumor formation by controlling the progression of mitosis. The expression of both long(170 kDa) and short lats1 isoforms(120 kDa) in vertebrate retinal cells raises the possibility that these lats1 proteins may act as negative key regulators of the cell cycle each of them performing a unique role (PMID:15777619). In mammalian cells, LATS1 was phosphorylated in a cell cycle-dependent manner and complexed with CDC2 in early mitosis (PMID:9988268). LATS1 also can be detected as 120 kDa and 140-150 kDa, and play a key role in the regulation of Hippo pathway (PMID: 27940445).