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LAYN Rekombinanter Antikörper

LAYN Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250175B7

Kat-Nr. : 85900-1-PBS

Synonyme

Layilin, UNQ208/PRO234

Filter:
  • Western Blot (WB) analysis of various lysates using LAYN Recombinant monoclonal antibody (85900-1-RR)

    WB analysis using 85900-1-RR (same clone as 85900-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85900-1-RR (LAYN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85900-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LAYN Recombinant monoclonal antibody (85900-1-RR)

    IHC staining of mouse brain using 85900-1-RR (same clone as 85900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85900-1-RR (LAYN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85900-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using LAYN Recombinant monoclonal antibody (85900-1-RR)

    IF Staining of A549 using 85900-1-RR (same clone as 85900-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using LAYN antibody (85900-1-RR, Clone: 250175B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85900-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85900-1-RR

    Affinity and Kinetic Characterization of 85900-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85900-1-RR against Human LAYN were performed. The affinity constant is 7.21 nM.

Geprüfte Anwendungen

Produktinformation

85900-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA LAYN und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen LAYN fusion protein Ag14508
Vollständiger Name layilin
Berechnetes Molekulargewicht 382 aa, 43 kDa
Beobachtetes Molekulargewicht43 kDa
GenBank-ZugangsnummerBC025407
Gene symbol LAYN
Gene ID (NCBI) 143903
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LAYN acts as a receptor for hyaluronic acid (HA) and is involved in various cellular processes, including cell adhesion, movement, and signal transduction. In the context of cancer, LAYN has been identified as a prognostic biomarker. High expression of LAYN is associated with poor prognosis and increased immune cell infiltration in various cancers, such as colorectal cancer and gastric cancer. It may also play a role in T-cell exhaustion and the regulation of tumor-associated macrophages (PMID: 30761122).